Separation, Purification and Structural Analysis of Flavonoids from Diospyros kaki Leaves

doi:10.7506/spkx1002-6630-201102014

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 57-61.doi: 10.7506/spkx1002-6630-201102014

• Processing Technology • Previous Articles Next Articles

Separation, Purification and Structural Analysis of Flavonoids from Diospyros kaki Leaves

SUN Hua-peng1，ZHONG Xiao-hong1,*，LU Ying1,2，ZHOU Zhou1

1. College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China；
2. Hunan Provincial Research Center of Natural Products, Hunan Agricultural University, Changsha 410128, China

Received:2010-04-13 Revised:2010-12-09 Online:2011-01-25 Published:2011-01-10
Contact: ZHONG Xiao-hong E-mail:xh-zhong@163.com

Abstract

Abstract:

In this study, high-speed countercurrent chromatography (HSCCC) followed by preparative high performance liquid chromatography (HPLC) was used to separate and purify flavonoid monomers from the crude ethyl acetate extract from Diospyros kaki leaves. Meanwhile, the flavonoid monomers obtained were preliminarily identified by color development reaction and further structurally characterized by UV spectroscopy and mass spectroscopy. The HSCCC separation of flavonoids was achieved by using a solvent system composed of ethylacetate, methanol and water (5:1:5, V/V) at a flow rate of 1.5 mL/min and setting the rotational speed at 850 r/min with a detection wavelength set at 254 nm. For further separation, the separated fractions were then applied to preparative HPLC, and 4 flavonoid compounds, named A-Ⅱ, A-Ⅲ, B-Ⅱ and B-Ⅲ, were obtained. A-II and A-III might be quercetin flavonoids, and B-Ⅱ and B-Ⅲ might be kaemoferol.

Key words: Diospyros kaki leaves, flavonoids, HSCCC, preparative HPLC, structural identification

CLC Number:

R931.6

SUN Hua-peng1，ZHONG Xiao-hong1,*，LU Ying1,2，ZHOU Zhou1. Separation, Purification and Structural Analysis of Flavonoids from Diospyros kaki Leaves[J]. FOOD SCIENCE, 2011, 32(2 ): 57-61.

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Separation, Purification and Structural Analysis of Flavonoids from Diospyros kaki Leaves

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